Furthermore, the physical proximity of the cells in the biofilm favors the acquisition of resistance through genetically transmissible elements. This increased antibiotic resistance is mainly due to the limited diffusion of drugs through the biofilm matrix and to physiological changes in bacteria due to the environmental conditions featuring the biofilm. Compared to their planktonic counterparts, biofilm-associated bacteria exhibit greater resistance to antibiotic agents. Our results highlighted that Sulfamides and Glycopeptides for the major Gram-positive strains and Fluoroquinolones, Carbapenems, and Aminoglycosides for the most represented Gram-negative isolates could be the most suitable therapeutic choice for most biofilm-DRIs.īiofilm is a structured bacterial community, enclosed in a self-produced polymeric matrix and adhered to biotic or abiotic surfaces. In contrast, among non-biofilm producers, only 11.8% were classified as MDR strains. All moderate and strong biofilm producers and 81% of weak biofilm producers were Multidrug Resistance (MDR) strains. All biofilm producing isolates were sensitive to a limited spectrum of antibiotic classes. Among 78 bacterial strains, 43.6% were non-biofilm producers while 56.4% produced biofilms. Among the 94 MDs isolated strains, 42.7% were Gram-positive, 40.3% Gram-negative, and 17% Candida species. Biofilm-forming abilities were assessed using the tissue culture plate (TCP) method. Identification of isolates and antibiotic susceptibility testing were performed using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and Phoenix Becton Dickinson, respectively. The study was conducted on 76 MDs, collected at University Hospital of Campania “Luigi Vanvitelli,” between October 2019 and September 2020. The present study aimed to detect biofilm formation among MD bacterial isolates and to explore their antibiotic resistance profile. Resistant DRIs are mainly due to the MDs contamination by bacteria producing biofilm. Patients with DRIs often have negative outcomes following the failure of antibiotic treatment. Prolonged periods of implantation carry a high risk of device-related infections (DRIs). Microbial biofilms pose a serious threat to patients requiring medical devices (MDs).
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